Fake “Frank” Lu, Ph.D.
Instructor in Neurosurgery
Harvard Medical School Brigham and Women’s Hospital
“Transforming Coherent Raman Scattering Microscopy for Label-Free Biomolecular Imaging Toward Clinical Applications”
Label-free optical microscopy is an emerging technology in biology and medicine, to perform live imaging without affecting cell functions, or in vivo imaging of human with no fluorescent staining. While Raman spectroscopy has been used for molecular characterization for cancer detection with high sensitivity, it is not good at imaging due to the ultraweak signals. By coherently exciting and detecting the chemical bond vibration in molecules, coherent Raman scattering (CRS) microscopy, with two modalities of coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS), has been used for label-free imaging of various biomolecules or drugs with high spatiotemporal resolution and high chemical specificity.
In this seminar, I will first review recent technical efforts that have greatly improved CRS imaging speed, detection sensitivity and spectral acquisition, and I will further present several label-free biomolecular imaging applications of the technology. In particular, transforming SRS microscopy for clinical use is a major direction of my research. The methodology of SRS label-free histopathology for rapid cancer detection and image-guided precision surgery has shown a great potential to impact on the current clinical practices of pathology and surgery.
The goal of my future research is to establish a new biophotonics program focusing on advanced coherent Raman and multiphoton imaging of single living cells, in vivo animals and fresh human tissue at the most translational frontier for neuro-oncology and neuroscience studies with extensive collaborations in the area.
Dr. Frank Lu received his Bachelor degree in Optoelectronics and Master degree in Optical Engineering from Zhejiang University in China in 2003 and 2006, respectively, and PhD in Biomedical Engineering from the National University of Singapore in 2010.
Dr. Lu completed his initial postdoctoral training at Harvard University with Professor Sunney Xie (2011-2014). He is currently an Instructor in Neurosurgery at Harvard Medical School and Brigham and Women’s Hospital (2014-present). His present research on label-free brain tumor imaging with stimulated Raman scattering (SRS) microscopy is funded by an NIH K99/R00 Pathway to Independence Grant (2015-2020), with mentors Drs. Alexandra Golby, Nathalie Agar, and Sunney Xie. Dr. Lu’s decade-long research has been focused on coherent Raman imaging for biological, biomedical and translational applications.